34 & 35. Proteomics and Macromolecular Crystallography (PMCSR)
At Proteomic and Macromolecular Crystallography Shared Resource (PMCSR), we provide the following services:
- Discovery-based and targeted proteomic analysis: The facility provides cancer researchers access to state-of-the-art mass spectrometers and software for qualitative (e.g. protein identification), quantitative (e.g. relative quantification), and post-translational modification (PTM) analyses. The services include i) mass determination of purified proteins/peptides, ii) identification of purified protein complexes, iii) site targeted PTM mapping on purified proteins, targeted MRM quantification, iv) global proteome analysis, subproteome isolation and identification, and v) large-scale PTM characterization. The facility director and dedicated technical personnel have extensive expertise in mass spectrometry-based techniques and will provide consultation on experimental design, data analysis, and troubleshooting. We encourage researchers to contact us prior to submission of samples to discuss sample preparation, experimental design, and potential outcomes of research projects.
- Crystallography: we crystallize and determine structures of macromolecules (protein, DNA, and RNA). These structures provide information for protein-ligand interactions, protein-protein or protein-nucleic-acid complexes. This information helps in elucidation of disease mechanisms and development of effective drugs. We also perform epitope-engineering so as to make the macromolecule amenable to crystal nucleation. We can produce a model for a protein with unknown structure, if its structural homolog with >30% sequence identity is available in PDB.